Iniciation and cultivation of multipotent mesenchimal human umbilical stroma cells in a laboratory experiment

BACKGROUND: Whartons jelly of the human umbilical cord is one sources multipotent mesenchymal stromal cells. The cell population obtained from postpartum biomaterial characterized by high proliferative and regenerative properties. Isolation a culture cells does not pose threat to health life donor.
 AIM: Optimization technique for isolating reproducible an urgent task in biomedicine, which can accelerate process obtaining donor therapy tissue engineering.
 MATERIALS AND METHODS: In study, main techniques methods stroma were tested, cultivation was optimized increase its efficiency reduce time growth biomass. effect components nutrient medium on studied. Currently, there no universal composition medium; various studies, media different manufacturers are used, differ composition. most discussed issue selection serum, part medium.
 RESULTS: work, comparative evaluation five sera carried out. It has been shown that stable physiological parameters observed suspension samples with addition FBS (SKPK, Russia) (Capricorn, USA) sera. A study hypoxia combination effective showed hypoxic stress acts as activator primary proliferation. assessment serum out visually using microscopy, changes morphology during cultivation, results testing action intensity respiration free immobilized under inhibitors.
 CONCLUSION: As result experiments, influence type initiation expansion explants further proliferation vitro established. Hypoxia exposure enhances fragments tissue.